Part:BBa_K277045
3L.3_23.B2.05
3L.3_23.B2.05 is 750 bases long and is cloned into the pGem-T vector.
3L.3_23.B2.05 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.B2.05 is a constituent of 3L.3_23.B2 (along with 3L.3_23.B2.01, 3L.3_23.B2.02, 3L.3_23.B2.03, 3L.3_23.B2.04, 3L.3_23.B2.06, 3L.3_23.B2.07, 3L.3_23.B2.08, 3L.3_23.B2.09, 3L.3_23.B2.10, 3L.3_23.B2.11, and 3L.3_23.B2.12.)
This part contains at least part of the following features (positions offset from first base of sequence):
kind and name offset notes
mutation_affecting_coding_sequence YCL048W_re_remove_BpuEI (596..604) removal of BpuEI
gene YCL048W (-151..+1240) Protein of unknown function%2C redundant with Sps2p for the organization of the beta-glucan layer of the spore wall
forward_primer YCL048W_tagf1v1 (178..205)
reverse_primer YCL048W_tagr1v1 (625..652)
mutation_affecting_coding_sequence YCL048W_re_remove_Bpu10I (569..577) removal of Bpu10I
Sequence (the first 750 bases correspond to coordinates 32588..33337 in synthetic chromosome yeast_chr3_3_23)
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 128
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 128
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 128
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 128
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 128
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 277
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